Fatchiyah F
Brawijaya University, Indonesia
Title: Caprine milk alpha-S2 casein protein of ethawah breeds is able to enhance biological activities related with gene susceptibility of human disease regulation
Biography
Biography: Fatchiyah F
Abstract
Most of biological signaling pathway was orchestrated at the level of proteins and biological active compounds, that both molecules recognition have ability to interact and modulate molecular mechanisms underlying an organism’s physiological functions. Differentiation of structural and physicochemical properties of bioactive compounds is important factor to determine the biological function. Local Indonesian goat Ethawah breed milk is one of nutritionally enriched dairy breed milk to provide biological function modulation without health risks. Recently our study, we found the high amount of caprine alpha-S2 casein of goat Ethawah breed milk exclusively isolated from 36kDa, and identified the eight of bioactive peptides fragments of caprine alpha-S2 casein by MALDI-TOF analysis. This study focuses on elucidating the biological function of caprine milk alpha-S2 casein protein provides by in vivo, in vitro and in silico analyses. We found that alpha-S2 casein protein in high MG environment inhibits the decreasing of viability due to increasing the proliferation of MC3T3E1pre-osteoblast cell. In animal model, this protein able to down regulated cytokine pro-inflammation on some tissues target and controlled RAGE-AGE signaling pathway related with ROS and oxidative stress. These data also confirmed by in silico analysis, we detected each of bioactive peptide of caprine milk alpha-S2 casein protein has display a wide range of physiological functions as anti-inflammation, anti-oxidative, and immunomodulatory mechanism to regulate the cellular and molecular signaling mechanism to promote health life improvement.