Yolanda B. De Rijke
Erasmus MC: University Medical Center Rotterdam, The Netherlands
Title: New CgA assay of Thermo Fischer in the monitoring of NET patient
Biography
Biography: Yolanda B. De Rijke
Abstract
Chromogranin A (CgA) is used as an aid in the diagnosis and follow-up of neuroendocrine tumors. We evaluated the ThermoFisher Brahms CgA Kryptor assay against the CisBio assay. Recently, we evaluated also the new generation CgA assay (CgA II). Methods: Analytical validation of the CgA II assay was performed and included precision, linearity and recovery studies as well as a comparison study with the current CgA assay and a stability study. For the stability study, individual serum samples were aliquoted and stored at different storage temperatures (room temperature, 4â°C and -20â°C) until assayed. Results: The CgA II assay showed a good correlation with the current CgA assay. Although the current assay was not stable at 4°C, the CgA II assay is stable at 4°C for at least 48 hr and therewith comparable to the stability we used to with the CisBio assay. Conclusion: Our study showed that the CisBio assay can conveniently be replaced by the Kryptor assay which is a robust assay with good performance. The CgA II assay uses antibodies directed against different epitopes and the results have demonstrated that this epitope is stable over time and at different temperatures, including 4°C.